Mouse Anti-SOX9 Recombinant Antibody (clone ABT-SOX9)
CAT#: ZG-0104C
This product is a mouse antibody that recognizes SOX9. It can be used in a wide variety of applications, such as: IHC-P, IF.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-SOX9 Antibody (clone ABT-SOX9) in IHC
Immunohistochemical analysis of paraffin-embedded Colon Carcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 2 Mouse Anti-SOX9 Antibody (clone ABT-SOX9) in IHC
Immunohistochemical analysis of paraffin-embedded Colon Carcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
WB
Figure 3 Mouse Anti-SOX9 Antibody (clone ABT-SOX9) in WB
Immunohistochemical analysis of paraffin-embedded Colon Carcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22456/if_selected.jpg
Normal Tissue
Figure 2 Salivary gland.
High expression in salivary gland
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_B_1_1_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity: 75%-25% Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_5_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (AN3-CA, Hep G2, OE19, U-251 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125398-SOX9
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Specifications
- Immunogen
- Synthesized peptide derived from human SOX9
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- SOX9
- Species Reactivity
- Human, Mouse
- Clone
- ABT-SOX9
- Applications
- IHC-P, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunohistochemistry-Paraffin, Immunofluorescence. For details, refer to Tested Data.
Target
REVIEWS AND Q&AS
CITATIONS
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Precision in Detection
Using the Mouse Anti-SOX9 Antibody, we've experienced unparalleled precision in detecting SOX9 protein levels in our cancer research. It's significantly enabled us to differentiate expression patterns in tumor versus normal tissues, aiding our understanding of SOX9's impact on disease progression. The antibody's robustness ensures consistent results across our various immunohistochemical assays.
Reliable Performance in IHC
The Mouse Anti-SOX9 Recombinant Antibody delivers exceptional performance in IHC applications, allowing detailed visualization of SOX9 expression in tissue samples. Its sensitivity ensures even low-level expression is detected, which has been pivotal in advancing our cancer biomarker research. It's truly a reliable asset for any research on protein localization.
Facilitates Cancer Research
This antibody has opened new avenues in our cancer research, allowing precise localization and quantification of SOX9. Its effectiveness in immunofluorescent and immunohistochemical applications means we can closely monitor its involvement in tumor biology, paving the way for potential therapeutic insights.
Q&As
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What are the specific benefits of using the Mouse Anti-SOX9 Recombinant Antibody for studying protein interactions?
A: By binding specifically to the SOX9 protein, this antibody allows researchers to investigate its interactions with other cellular proteins. This is crucial for decoding signaling pathways and regulatory networks where SOX9 is involved, providing valuable insights into transcriptional regulation and gene expression patterns.
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What applications is the Mouse Anti-SOX9 Recombinant Antibody particularly suited for in research?
A: This antibody is ideal for applications in Immunohistochemistry-Paraffin (IHC-P) and Immunofluorescence (IF). It enables the detailed visualization of SOX9 expression in tissue and cellular samples, aiding in developmental biology studies and oncological research by mapping protein localization and abundance under various physiological and pathological conditions.
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What are the targeted interactions of SOX9 that can be studied using this antibody?
A: By enabling the detection of SOX9 in conjunction with other proteins, this antibody allows researchers to study its interaction with transcription factors and co-regulators. This insight is crucial to understanding the regulatory mechanisms governing tissue-specific gene expression.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# ZG-0104C, RRID: AB_3112063)
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Related Signaling Pathways
cAMP Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SOX9"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3882z | Mouse Anti-SOX9 Recombinant Antibody (clone 15C2) | WB, ICC, IF, ELISA | Mouse IgG1 |
| MOB-1914CT | Recombinant Mouse anti-Human SOX9 Monoclonal antibody (3C10) | ELISA, ICC, IF, IHC-P, WB | |
| ZG-0122U | Rabbit Anti-SOX9 Recombinant Antibody (clone 5H12) | ELISA, IHC | Rabbit IgG |
| VS3-XY1457 | Mouse Anti-SOX9 Recombinant Antibody (clone 1B11) | ELISA, WB, ICC | Mouse IgG1 |
| VS3-FY1631 | Rabbit Anti-SOX9 Recombinant Antibody (clone R06-7C6) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3378 | Hi-Affi™ Recombinant Rabbit Anti-SOX9 Monoclonal Antibody (DS3378AB) | WB, ICC, IF, IP | IgG |
| MOR-0106-FY | Rabbit Anti-SOX9 Recombinant Antibody (clone AFY0077) | ICC, IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1045 | Mouse Anti-SOX9 Recombinant Antibody (clone 334CT39.1.4) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1099 | CytoStream™ Rabbit Anti-SOX9 Recombinant Antibody (VS13-YC1099) | WB, ICC, IF, IHC-P, IP, FC, ChIP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6830 | Anti-SOX9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6831 | Anti-Mouse SOX9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6832 | Anti-Rat SOX9 Immunohistochemistry Kit | IHC |
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